## ----message=FALSE, include=FALSE, results="hide", setup, echo=FALSE----------
knitr::opts_chunk$set(echo = TRUE,
                      eval = TRUE,
                      message = FALSE,
                      warning = FALSE,
                      collapse = TRUE,
                      tidy = FALSE,
                      cache = FALSE,
                      dev = "png",
                      comment = "#>")
library(rbioapi)
rba_options(timeout = 600)

## ----rba_reactome_analysis----------------------------------------------------
## 1 We create a simple vector with our genes
genes <- c("p53", "BRCA1", "cdk2", "Q99835", "CDC42", "CDK1", "KIF23", "PLK1", "RAC2", "RACGAP1", "RHOA", "RHOB", "MSL1", "PHF21A", "INSR", "JADE2", "P2RX7", "CCDC101", "PPM1B", "ANAPC16", "CDH8", "HSPA1L", "CUL2", "ZNF302", "CUX1", "CYTH2", "SEC22C", "EIF4E3", "ROBO2", "CXXC1", "LINC01314", "ATP5F1")

## 2 We call reactome analysis with the default parameters
analyzed <- rba_reactome_analysis(input = genes,
                                 projection = TRUE,
                                 p_value = 0.01)

## 3 As always, we use str() to inspect the resutls
str(analyzed, 1)

## 4 Note that in the summary element:
### 4.a because we provided a simple vector, the analysis type was: over-representation
### 4.b You need the token for other rba_reactome_analysis_* functions
str(analyzed$summary)

## 5 Analsis results are in the pathways data frame:

## ----analysis_results, echo=FALSE---------------------------------------------
DT::datatable(data = jsonlite::flatten(analyzed$pathways),
              options = list(scrollX = TRUE, 
                             paging = TRUE,
                             fixedHeader = TRUE,
                             keys = TRUE,
                             pageLength = 5))


## ----rba_reactome_analysis_pdf/download, eval=FALSE---------------------------
#  # download a full pdf report
#  rba_reactome_analysis_pdf(token = analyzed$summary$token,
#                            species = 9606)
#  # download the result in compressed json.gz format
#  rba_reactome_analysis_download(token = analyzed$summary$token,
#                                 request = "results",
#                                 save_to = "reactome_results.json")

## ----rba_reactome_analysis_import, eval=FALSE---------------------------------
#  re_uploaded <- rba_reactome_analysis_import(input = "reactome_results.json")

## ----rba_reactome_query_ex1---------------------------------------------------
## 1 query a pathway Entry
pathway <- rba_reactome_query(ids = "R-HSA-109581", enhanced = TRUE)
## 2 As always we use str() to inspect the output's structure
str(pathway, 2)



## 3 You can compare it with the webpage of R-HSA-202939 entry:
# https://reactome.org/content/detail/R-HSA-202939

## ----rba_reactome_query_ex2---------------------------------------------------
## 1 query a protein Entry
protein <- rba_reactome_query(ids = 66247, enhanced = TRUE)
## 2 As always we use str() to inspect the output's structure
str(protein, 1)



## 3 You can compare it with the webpage of R-HSA-202939 entry:
# https://reactome.org/content/detail/R-HSA-202939


## ----rba_reactome_xref--------------------------------------------------------
## 1 We provide HGNC ID to find what is the corresponding database ID in Reactome
xref_protein <- rba_reactome_xref("CD40")
## 2 As always use str() to inspect the output's structure
str(xref_protein, 1)

## ----xref_mapping-------------------------------------------------------------
## 1 Again, consider CD40 protein:
xref_mapping <- rba_reactome_mapping(id = "CD40",
                                    resource = "hgnc",
                                    map_to = "pathways")

## ----xref_mapping_df, echo=FALSE----------------------------------------------
DT::datatable(data = xref_mapping,
              options = list(scrollX = TRUE, 
                             paging = TRUE,
                             fixedHeader = TRUE,
                             keys = TRUE,
                             pageLength = 10))

## ----sessionInfo, echo=FALSE--------------------------------------------------
sessionInfo()

